CYBB ELISA Kit

Catalog No. ABIN5653479

Product Details CYBB ELISA Kit

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cytochrome b-245 Beta Polypeptide (CYBb). No significant cross-reactivity or interference between Cytochrome b-245 Beta Polypeptide (CYBb) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome b-245 Beta Polypeptide (CYBb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome b-245 Beta Polypeptide (CYBb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome b-245 Beta Polypeptide (CYBb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome b-245 Beta Polypeptide (CYBb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome b-245 Beta Polypeptide (CYBb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome b-245 Beta Polypeptide (CYBb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CYBB

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Alternative Name: Cytochrome b-245 Beta Polypeptide (CYBB Products)

Synonyms: zgc:64144 ELISA Kit, cgd ELISA Kit, gp91-1 ELISA Kit, gp91-phox ELISA Kit, gp91phox ELISA Kit, nox2 ELISA Kit, NOX2 ELISA Kit, cybb ELISA Kit, NOX1 ELISA Kit, AMCBX2 ELISA Kit, CGD ELISA Kit, GP91-1 ELISA Kit, GP91-PHOX ELISA Kit, GP91PHOX ELISA Kit, p91-PHOX ELISA Kit, Nox2 ELISA Kit, C88302 ELISA Kit, CGD91-phox ELISA Kit, Cgd ELISA Kit, Cyd ELISA Kit, Gp91-phox ELISA Kit, CYBB ELISA Kit, cytochrome b-245, beta polypeptide (chronic granulomatous disease) ELISA Kit, cytochrome b-245, beta polypeptide ELISA Kit, cytochrome b-245 heavy chain ELISA Kit, cytochrome b-245, beta polypeptide L homeolog ELISA Kit, cytochrome b-245 beta polypeptide ELISA Kit, cytochrome b-245 beta chain ELISA Kit, NADPH oxidase heavy chain subunit ELISA Kit, cybb ELISA Kit, CYBB ELISA Kit, cybb.L ELISA Kit, Cybb ELISA Kit, GP91-PHOX ELISA Kit

Background:

Gene Name: Cytochrome b-245 Beta Polypeptide

Gene Aliases: CGD, GP91PHOX, NOX2, Neutrophil cytochrome b91, Cytochrome b558 beta, Heme-binding membrane glycoprotein gp91phox, Superoxide-generating NADPH oxidase heavy chain

Gene ID: 1536

UniProt: P04839