CDA ELISA Kit

Catalog No. ABIN5653477

Product Details CDA ELISA Kit

Target: CDA (Cytidine Deaminase (CDA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cytidine Deaminase (CDA). No significant cross-reactivity or interference between Cytidine Deaminase (CDA) and analogues was observed.

Sensitivity: 0.32 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytidine Deaminase (CDA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytidine Deaminase (CDA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytidine Deaminase (CDA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytidine Deaminase (CDA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytidine Deaminase (CDA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytidine Deaminase (CDA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CDA

Target: CDA (Cytidine Deaminase (CDA))

Alternative Name: Cytidine Deaminase (CDA Products)

Synonyms: CDA ELISA Kit, cda ELISA Kit, zgc:77948 ELISA Kit, DDBDRAFT_0188181 ELISA Kit, DDBDRAFT_0230205 ELISA Kit, DDB_0188181 ELISA Kit, DDB_0230205 ELISA Kit, CDD ELISA Kit, 2210401N16Rik ELISA Kit, cytidine deaminase ELISA Kit, cytidine aminohydrolase; CDA ELISA Kit, cytidine deaminase b ELISA Kit, Cytidine deaminase ELISA Kit, cytidine deaminase Cdd ELISA Kit, CDA ELISA Kit, cda ELISA Kit, VNG_RS03500 ELISA Kit, OE_RS03485 ELISA Kit, CDD1 ELISA Kit, cdd ELISA Kit, cdab ELISA Kit, cdd-1 ELISA Kit, cdd-2 ELISA Kit, CNC04940 ELISA Kit, Tb09.160.1680 ELISA Kit, Cda ELISA Kit

Background:

Gene Name: Cytidine Deaminase

Gene Aliases: CDD, Cytidine aminohydrolase

Gene ID: 978

UniProt: P32320