CSRP1 ELISA Kit

Catalog No. ABIN5653462

Product Details CSRP1 ELISA Kit

Target: CSRP1 (Cysteine and Glycine-Rich Protein 1 (CSRP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cysteine And Glycine Rich Protein 1 (CSRP1). No significant cross-reactivity or interference between Cysteine And Glycine Rich Protein 1 (CSRP1) and analogues was observed.

Sensitivity: 0.057 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine And Glycine Rich Protein 1 (CSRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine And Glycine Rich Protein 1 (CSRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine And Glycine Rich Protein 1 (CSRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteine And Glycine Rich Protein 1 (CSRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteine And Glycine Rich Protein 1 (CSRP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteine And Glycine Rich Protein 1 (CSRP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CSRP1

Target: CSRP1 (Cysteine and Glycine-Rich Protein 1 (CSRP1))

Alternative Name: Cysteine And Glycine Rich Protein 1 (CSRP1 Products)

Synonyms: CRP ELISA Kit, CRP1 ELISA Kit, CSRP ELISA Kit, CYRP ELISA Kit, D1S181E ELISA Kit, AA408841 ELISA Kit, AA959891 ELISA Kit, AW545626 ELISA Kit, Csrp ELISA Kit, cb796 ELISA Kit, csrp1 ELISA Kit, zgc:77332 ELISA Kit, MGC85577 ELISA Kit, MGC89932 ELISA Kit, CSRP1 ELISA Kit, zgc:158737 ELISA Kit, cysteine and glycine rich protein 1 ELISA Kit, cysteine and glycine-rich protein 1 ELISA Kit, cysteine and glycine-rich protein 1a ELISA Kit, cysteine and glycine rich protein 1 L homeolog ELISA Kit, cysteine and glycine-rich protein 1b ELISA Kit, Cysteine and glycine-rich protein 1 ELISA Kit, CSRP1 ELISA Kit, Csrp1 ELISA Kit, csrp1a ELISA Kit, csrp1.L ELISA Kit, csrp1 ELISA Kit, csrp1b ELISA Kit, LOC100304834 ELISA Kit, Tsp_01053 ELISA Kit

Background:

Gene Name: Cysteine And Glycine Rich Protein 1

Gene Aliases: CRP1, CRP, CSRP, CYRP, HEL-141, Cysteine-rich protein 1, Epididymis luminal protein 141

Gene ID: 1465

UniProt: P21291