CYLD ELISA Kit

Catalog No. ABIN5653439

Product Details CYLD ELISA Kit

Target: CYLD (Cylindromatosis (Turban Tumor Syndrome) (CYLD))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cylindromatosis (CYLD). No significant cross-reactivity or interference between Cylindromatosis (CYLD) and analogues was observed.

Sensitivity: 0.115 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cylindromatosis (CYLD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cylindromatosis (CYLD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cylindromatosis (CYLD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cylindromatosis (CYLD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cylindromatosis (CYLD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cylindromatosis (CYLD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CYLD

Target: CYLD (Cylindromatosis (Turban Tumor Syndrome) (CYLD))

Alternative Name: Cylindromatosis (CYLD Products)

Synonyms: BRSS ELISA Kit, CDMT ELISA Kit, CYLD1 ELISA Kit, CYLDI ELISA Kit, EAC ELISA Kit, MFT ELISA Kit, MFT1 ELISA Kit, SBS ELISA Kit, TEM ELISA Kit, USPL2 ELISA Kit, 2010013M14Rik ELISA Kit, 2900009M21Rik ELISA Kit, C130039D01Rik ELISA Kit, mKIAA0849 ELISA Kit, cyld ELISA Kit, LRRGT00003 ELISA Kit, Rp1 ELISA Kit, Rp1h ELISA Kit, CYLD lysine 63 deubiquitinase ELISA Kit, cylindromatosis (turban tumor syndrome), a ELISA Kit, CYLD ELISA Kit, Cyld ELISA Kit, cylda ELISA Kit

Background:

Gene Name: Cylindromatosis

Gene Aliases: CDMT, CYLD1, CYLDI, EAC, USPL2, Turban Tumor Syndrome, Ubiquitin Carboxyl-Terminal Hydrolase CYLD, Ubiquitin Specific Peptidase Like 2, Deubiquitinating enzyme CYLD

Gene ID: 1540

UniProt: Q9NQC7

Pathways: Apoptosis, Activation of Innate immune Response