GJA4 ELISA Kit

Catalog No. ABIN5653301

Product Details GJA4 ELISA Kit

Target: GJA4 (Gap Junction Protein, alpha 4, 37kDa (GJA4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Connexin 37 (CX37). No significant cross-reactivity or interference between Connexin 37 (CX37) and analogues was observed.

Sensitivity: 0.061 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Connexin 37 (CX37). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Connexin 37 (CX37). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Connexin 37 (CX37), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Connexin 37 (CX37) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Connexin 37 (CX37) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Connexin 37 (CX37) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GJA4

Target: GJA4 (Gap Junction Protein, alpha 4, 37kDa (GJA4))

Alternative Name: Connexin 37 (GJA4 Products)

Synonyms: AU020209 ELISA Kit, AW558810 ELISA Kit, Cnx37 ELISA Kit, Cx37 ELISA Kit, Gja-4 ELISA Kit, CXN37 ELISA Kit, CX37 ELISA Kit, Cx39 ELISA Kit, ggCx39 ELISA Kit, cx41 ELISA Kit, gja4 ELISA Kit, gap junction protein alpha 4 ELISA Kit, gap junction protein, alpha 4 ELISA Kit, gap junction protein, alpha 4, 37kDa ELISA Kit, gap junction protein alpha 4 L homeolog ELISA Kit, GJA4 ELISA Kit, Gja4 ELISA Kit, gja4.L ELISA Kit

Background:

Gene Name: Connexin 37

Gene Aliases: GJA4, GJ-A4, Gap Junction Protein Alpha 4

Gene ID: 2701

UniProt: P35212

Pathways: Cell-Cell Junction Organization