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CD35 ELISA Kit

CR1 Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum
Catalog No. ABIN5653283
  • Target See all CD35 (CR1) ELISA Kits
    CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))
    Reactivity
    • 5
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.25 pg/mL - 2000 pg/mL
    Minimum Detection Limit
    31.25 pg/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Complement Receptor 1, Erythrocyte (CR1). No significant cross-reactivity or interference between Complement Receptor 1, Erythrocyte (CR1) and analogues was observed.
    Sensitivity
    12.9 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Receptor 1, Erythrocyte (CR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Receptor 1, Erythrocyte (CR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Receptor 1, Erythrocyte (CR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Receptor 1, Erythrocyte (CR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Receptor 1, Erythrocyte (CR1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Receptor 1, Erythrocyte (CR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all CD35 (CR1) ELISA Kits
    CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))
    Alternative Name
    Complement Receptor 1, Erythrocyte (CR1 Products)
    Synonyms
    C3BR ELISA Kit, C4BR ELISA Kit, CD35 ELISA Kit, KN ELISA Kit, C3DR ELISA Kit, CD21 ELISA Kit, Cr-1 ELISA Kit, Cr-2 ELISA Kit, Cr1 ELISA Kit, CR1-L ELISA Kit, CR2 ELISA Kit, CR1 ELISA Kit, complement C3b/C4b receptor 1 (Knops blood group) ELISA Kit, complement receptor 2 ELISA Kit, complement component (3d/Epstein Barr virus) receptor 2 ELISA Kit, complement receptor type 1 ELISA Kit, CR1 ELISA Kit, Cr2 ELISA Kit, CR2 ELISA Kit, LOC718120 ELISA Kit
    Background

    Gene Name: Complement Receptor 1, Erythrocyte

    Gene Aliases: CD35, C3BR, KN, C3b/C4b Receptor,Including Knops Blood Group System, Immune Adherence Receptor

    Gene ID
    1378
    UniProt
    P17927
    Pathways
    Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity
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