Adipsin ELISA Kit

Catalog No. ABIN5653268

Product Details Adipsin ELISA Kit

Target: Adipsin (CFD) (Complement Factor D (CFD))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Complement Factor D (CFD). No significant cross-reactivity or interference between Complement Factor D (CFD) and analogues was observed.

Sensitivity: 0.069 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor D (CFD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor D (CFD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor D (CFD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor D (CFD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor D (CFD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor D (CFD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Adipsin

Target: Adipsin (CFD) (Complement Factor D (CFD))

Alternative Name: Complement Factor D (CFD Products)

Synonyms: ADIPSIN ELISA Kit, ADN ELISA Kit, DF ELISA Kit, PFD ELISA Kit, Adn ELISA Kit, Df ELISA Kit, EVE ELISA Kit, CFD ELISA Kit, adipsin ELISA Kit, pfd ELISA Kit, cfdl ELISA Kit, wu:fb61f12 ELISA Kit, zgc:109940 ELISA Kit, 10.5 ELISA Kit, 10.9 ELISA Kit, 14.10 ELISA Kit, 20.35 ELISA Kit, CG2328 ELISA Kit, Dmel\\CG2328 ELISA Kit, E(eve) ELISA Kit, Eve ELISA Kit, F ELISA Kit, V ELISA Kit, VI ELISA Kit, eve2 ELISA Kit, even ELISA Kit, l(2)46CFg ELISA Kit, l(2)46CFh ELISA Kit, l(2)46CFj ELISA Kit, l(2)46CFp ELISA Kit, l(2)46Ce ELISA Kit, l(2)46Cg ELISA Kit, complement factor D ELISA Kit, complement factor D (adipsin) ELISA Kit, complement factor D (adipsin) L homeolog ELISA Kit, even skipped ELISA Kit, CFD ELISA Kit, Cfd ELISA Kit, cfd.L ELISA Kit, cfd ELISA Kit, eve ELISA Kit

Background:

Gene Name: Complement Factor D

Gene Aliases: CF-D, DF, PFD, ADN, Adipsin, Properdin Factor D, C3 convertase activator

Gene ID: 1675

UniProt: P00746

Pathways: Complement System