Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Complement C4c CLIA Kit

C4c Reactivity: Human Chemiluminescent Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Catalog No. ABIN5653239
  • Target See all Complement C4c (C4c) products
    Complement C4c (C4c) (Complement Component C4c (C4c))
    Reactivity
    Human
    Detection Method
    Chemiluminescent
    Method Type
    Sandwich ELISA
    Detection Range
    3.12 ng/mL - 200 ng/mL
    Minimum Detection Limit
    3.12 ng/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Complement Component 4c (C4c). No significant cross-reactivity or interference between Complement Component 4c (C4c) and analogues was observed.
    Sensitivity
    0.13 ng/mL
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 - 3 h
    Plate
    Pre-coated
    Protocol
    The microplate provided in this kit has been pre-coated with an antibody specific to Complement Component 4c (C4c). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement Component 4c (C4c). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement Component 4c (C4c) level in the sample or standard.,
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 4c (C4c) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 4c (C4c) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all Complement C4c (C4c) products
    Complement C4c (C4c) (Complement Component C4c (C4c))
    Alternative Name
    Complement Component 4c (C4c Products)
    Background

    Gene Name: Complement Component 4c

You are here:
Support