C1S CLIA Kit

Catalog No. ABIN5653213

Product Details C1S CLIA Kit

Target: C1S (Complement Component 1, S Subcomponent (C1S))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 468.75 pg/mL - 30000 pg/mL

Minimum Detection Limit: 468.75 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Complement Component 1, S Subcomponent (C1s). No significant cross-reactivity or interference between Complement Component 1, S Subcomponent (C1s) and analogues was observed.

Sensitivity: 18.22 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Complement Component 1, S Subcomponent (C1s). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement Component 1, S Subcomponent (C1s). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement Component 1, S Subcomponent (C1s) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 1, S Subcomponent (C1s) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 1, S Subcomponent (C1s) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: C1S (Complement Component 1, S Subcomponent (C1S))

Alternative Name: Complement Component 1, S Subcomponent (C1S Products)

Synonyms: AA959438 CLIA Kit, AI255193 CLIA Kit, AI327365 CLIA Kit, C1sa CLIA Kit, r-gsp CLIA Kit, C1S CLIA Kit, complement C1s CLIA Kit, complement component 1, s subcomponent 1 CLIA Kit, complement component 1, s subcomponent CLIA Kit, complement component 1, s subcomponent L homeolog CLIA Kit, complement C1s subcomponent CLIA Kit, C1S CLIA Kit, C1s1 CLIA Kit, C1s CLIA Kit, c1s CLIA Kit, c1s.L CLIA Kit, LOC100558615 CLIA Kit

Background:

Gene Name: Complement Component 1, S Subcomponent

Gene Aliases: C1 esterase

Gene ID: 716

UniProt: P09871

Pathways: Complement System