CIRBP ELISA Kit

Catalog No. ABIN5653090

Product Details CIRBP ELISA Kit

Target: CIRBP (Cold Inducible RNA Binding Protein (CIRBP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cold Inducible RNA Binding Protein (CIRBP). No significant cross-reactivity or interference between Cold Inducible RNA Binding Protein (CIRBP) and analogues was observed.

Sensitivity: 29 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cold Inducible RNA Binding Protein (CIRBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cold Inducible RNA Binding Protein (CIRBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cold Inducible RNA Binding Protein (CIRBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cold Inducible RNA Binding Protein (CIRBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cold Inducible RNA Binding Protein (CIRBP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cold Inducible RNA Binding Protein (CIRBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CIRBP

Target: CIRBP (Cold Inducible RNA Binding Protein (CIRBP))

Alternative Name: Cold Inducible RNA Binding Protein (CIRBP Products)

Synonyms: CIRP ELISA Kit, Cirp ELISA Kit, R74941 ELISA Kit, Xcirp ELISA Kit, cirbp ELISA Kit, cirbp-a ELISA Kit, cirbp2 ELISA Kit, cirp ELISA Kit, cirp1 ELISA Kit, cirp2 ELISA Kit, xcirp2 ELISA Kit, XCIRP ELISA Kit, XCIRP-1 ELISA Kit, cirbp-b ELISA Kit, cirp-1 ELISA Kit, APBP-1 ELISA Kit, zgc:136559 ELISA Kit, CIRBP ELISA Kit, A18 hnRNP ELISA Kit, cold inducible RNA binding protein ELISA Kit, cold-inducible RNA-binding protein ELISA Kit, cold inducible RNA binding protein S homeolog ELISA Kit, cold inducible RNA binding protein L homeolog ELISA Kit, cold inducible RNA binding protein b ELISA Kit, CIRBP ELISA Kit, EDI_107320 ELISA Kit, Cirbp ELISA Kit, cirbp.S ELISA Kit, cirbp.L ELISA Kit, cirbp ELISA Kit, cirbpb ELISA Kit

Background:

Gene Name: Cold Inducible RNA Binding Protein

Gene Aliases: CIRP, A18HNRNP, Glycine-Rich RNA Binding Protein

Gene ID: 1153

UniProt: Q14011

Pathways: Ribonucleoprotein Complex Subunit Organization