Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

COQ10B ELISA Kit

COQ10B Reactivity: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum
Catalog No. ABIN5653074
  • Target See all COQ10B ELISA Kits
    COQ10B (Coenzyme Q10 Homolog B (COQ10B))
    Reactivity
    • 3
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.312 ng/mL - 20 ng/mL
    Minimum Detection Limit
    0.312 ng/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Coenzyme Q10 Homolog B (COQ10B). No significant cross-reactivity or interference between Coenzyme Q10 Homolog B (COQ10B) and analogues was observed.
    Sensitivity
    0.134 ng/mL
    Featured
    Discover our best selling COQ10B ELISA Kit
    Top Product
    Discover our top product COQ10B ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coenzyme Q10 Homolog B (COQ10B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coenzyme Q10 Homolog B (COQ10B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coenzyme Q10 Homolog B (COQ10B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coenzyme Q10 Homolog B (COQ10B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coenzyme Q10 Homolog B (COQ10B) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coenzyme Q10 Homolog B (COQ10B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all COQ10B ELISA Kits
    COQ10B (Coenzyme Q10 Homolog B (COQ10B))
    Alternative Name
    Coenzyme Q10 Homolog B (COQ10B Products)
    Synonyms
    1500041J02Rik ELISA Kit, 1700030I21Rik ELISA Kit, 9530077A17Rik ELISA Kit, AV002237 ELISA Kit, AW742641 ELISA Kit, RGD1359509 ELISA Kit, fc38f07 ELISA Kit, im:6912267 ELISA Kit, wu:fc38f07 ELISA Kit, zgc:112103 ELISA Kit, coenzyme Q10B ELISA Kit, COQ10B ELISA Kit, Coq10b ELISA Kit, coq10b ELISA Kit
    Background

    Gene Name: Coenzyme Q10 Homolog B

    Gene Aliases: Coenzyme Q-binding protein COQ10 homolog B, mitochondrial

    Gene ID
    80219
    UniProt
    Q9H8M1
You are here:
Support