CD8 alpha ELISA Kit

Catalog No. ABIN5653029

Product Details CD8 alpha ELISA Kit

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 8a (CD8a). No significant cross-reactivity or interference between Cluster Of Differentiation 8a (CD8a) and analogues was observed.

Sensitivity: 0.57 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 8a (CD8a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 8a (CD8a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 8a (CD8a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 8a (CD8a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 8a (CD8a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 8a (CD8a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: Cluster Of Differentiation 8a (CD8A Products)

Synonyms: cd8 ELISA Kit, LOC100136450 ELISA Kit, BB154331 ELISA Kit, Ly-2 ELISA Kit, Ly-35 ELISA Kit, Ly-B ELISA Kit, Lyt-2 ELISA Kit, zgc:136643 ELISA Kit, CD8 ELISA Kit, Leu2 ELISA Kit, MAL ELISA Kit, p32 ELISA Kit, RHACD8-4 ELISA Kit, CD8a molecule ELISA Kit, uncharacterized LOC100125537 ELISA Kit, CD8 alpha ELISA Kit, CD8 antigen, alpha chain ELISA Kit, T-cell surface glycoprotein CD8 alpha chain ELISA Kit, Cd8a ELISA Kit, LOC100125537 ELISA Kit, LOC100136450 ELISA Kit, CD8A ELISA Kit, cd8a ELISA Kit, LOC100126556 ELISA Kit

Background:

Gene Name: Cluster Of Differentiation 8a

Gene Aliases: p32, CD8-A, Leu2, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 925

UniProt: P01732

Pathways: TCR Signaling