CHAF1B ELISA Kit

Catalog No. ABIN5652941

Product Details CHAF1B ELISA Kit

Target: CHAF1B (Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Chromatin Assembly Factor 1, Subunit B (CHAF1B). No significant cross-reactivity or interference between Chromatin Assembly Factor 1, Subunit B (CHAF1B) and analogues was observed.

Sensitivity: 0.052 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chromatin Assembly Factor 1, Subunit B (CHAF1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chromatin Assembly Factor 1, Subunit B (CHAF1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chromatin Assembly Factor 1, Subunit B (CHAF1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chromatin Assembly Factor 1, Subunit B (CHAF1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chromatin Assembly Factor 1, Subunit B (CHAF1B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chromatin Assembly Factor 1, Subunit B (CHAF1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CHAF1B

Target: CHAF1B (Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B))

Alternative Name: Chromatin Assembly Factor 1, Subunit B (CHAF1B Products)

Synonyms: CHAF1B ELISA Kit, wu:fd07c09 ELISA Kit, wu:fe36g06 ELISA Kit, wu:fv38g09 ELISA Kit, zgc:56096 ELISA Kit, CAF-1 ELISA Kit, CAF-IP60 ELISA Kit, CAF1 ELISA Kit, CAF1A ELISA Kit, CAF1P60 ELISA Kit, MPHOSPH7 ELISA Kit, MPP7 ELISA Kit, 2600017H24Rik ELISA Kit, C76145 ELISA Kit, CAF-I p60 ELISA Kit, CAF-Ip60 ELISA Kit, caf-1 ELISA Kit, CAF-1P60 ELISA Kit, chromatin assembly factor 1 subunit B ELISA Kit, chromatin assembly factor 1, subunit B ELISA Kit, chromatin assembly factor 1, subunit B (p60) ELISA Kit, chromatin assembly factor 1 subunit B L homeolog ELISA Kit, CHAF1B ELISA Kit, chaf1b ELISA Kit, Chaf1b ELISA Kit, chaf1b.L ELISA Kit

Background:

Gene Name: Chromatin Assembly Factor 1, Subunit B

Gene Aliases: CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7, M-Phase Phosphoprotein 7, Chromatin Assembly Factor I,p60 subunit, M-phase phosphoprotein 7

Gene ID: 110749

UniProt: Q9D0N7