CGA ELISA Kit

Catalog No. ABIN5652937

Product Details CGA ELISA Kit

Target: CGA (Glycoprotein Hormones, alpha Polypeptide (CGA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 123.5 pg/mL - 10000 pg/mL

Minimum Detection Limit: 123.5 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Chorionic Gonadotropin Alpha Polypeptide (CGa). No significant cross-reactivity or interference between Chorionic Gonadotropin Alpha Polypeptide (CGa) and analogues was observed.

Sensitivity: 49.4 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Chorionic Gonadotropin Alpha Polypeptide (CGa) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Chorionic Gonadotropin Alpha Polypeptide (CGa) and unlabeled Chorionic Gonadotropin Alpha Polypeptide (CGa) (Standards or samples) with the pre-coated antibody specific to Chorionic Gonadotropin Alpha Polypeptide (CGa). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Chorionic Gonadotropin Alpha Polypeptide (CGa) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Chorionic Gonadotropin Alpha Polypeptide (CGa) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chorionic Gonadotropin Alpha Polypeptide (CGa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chorionic Gonadotropin Alpha Polypeptide (CGa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CGA

Target: CGA (Glycoprotein Hormones, alpha Polypeptide (CGA))

Alternative Name: Chorionic Gonadotropin Alpha Polypeptide (CGA Products)

Synonyms: CG-ALPHA ELISA Kit, FSHA ELISA Kit, GPHA1 ELISA Kit, GPHa ELISA Kit, HCG ELISA Kit, LHA ELISA Kit, TSHA ELISA Kit, CG-alpha ELISA Kit, GPHalpha ELISA Kit, Tsha ELISA Kit, aGSU ELISA Kit, alpha-GSU ELISA Kit, alphaGSU ELISA Kit, alphaSU ELISA Kit, CGA ELISA Kit, FSH-alpha ELISA Kit, LSH-alpha ELISA Kit, TSH-alpha ELISA Kit, cg-alpha ELISA Kit, cga ELISA Kit, cga-a ELISA Kit, cga-b ELISA Kit, fsha ELISA Kit, gpha ELISA Kit, gpha1 ELISA Kit, hcg ELISA Kit, lha ELISA Kit, tsha ELISA Kit, GTH-alpha ELISA Kit, gsu-alpha ELISA Kit, glycoprotein hormones, alpha polypeptide ELISA Kit, glycoprotein hormones, alpha subunit ELISA Kit, chromogranin A ELISA Kit, glycoprotein hormones, alpha polypeptide L homeolog ELISA Kit, CGA ELISA Kit, Cga ELISA Kit, CHGA ELISA Kit, cga.L ELISA Kit, cga ELISA Kit

Background:

Gene Name: Chorionic Gonadotropin Alpha Polypeptide

Gene Aliases: FSHA, GPHA1, GPHa, HCG, LHA, TSHA, Thyroid Stimulating Hormone Alpha, Follicle-Stimulating Hormone Alpha Subunit, Luteinizing hormone alpha chain, Lutropin alpha chain

Pathways: Metabolism of Steroid Hormones and Vitamin D, Thyroid Hormone Synthesis, Hormone Transport, Peptide Hormone Metabolism