CCR2 ELISA Kit

Catalog No. ABIN5652834

Product Details CCR2 ELISA Kit

Target: CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.12 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Chemokine C-C-Motif Receptor 2 (CCR2). No significant cross-reactivity or interference between Chemokine C-C-Motif Receptor 2 (CCR2) and analogues was observed.

Sensitivity: 34 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Receptor 2 (CCR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Receptor 2 (CCR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Receptor 2 (CCR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Receptor 2 (CCR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-C-Motif Receptor 2 (CCR2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-C-Motif Receptor 2 (CCR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CCR2

Target: CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))

Alternative Name: Chemokine C-C-Motif Receptor 2 (CCR2 Products)

Synonyms: CC-CKR-2 ELISA Kit, CCR-2 ELISA Kit, CCR2A ELISA Kit, CCR2B ELISA Kit, CD192 ELISA Kit, CKR2 ELISA Kit, CKR2A ELISA Kit, CKR2B ELISA Kit, CMKBR2 ELISA Kit, MCP-1-R ELISA Kit, ACKR5 ELISA Kit, CKRX ELISA Kit, CRAM ELISA Kit, CRAM-A ELISA Kit, CRAM-B ELISA Kit, HCR ELISA Kit, Cc-ckr-2 ELISA Kit, Ccr2a ELISA Kit, Ccr2b ELISA Kit, Ckr2 ELISA Kit, Ckr2a ELISA Kit, Ckr2b ELISA Kit, Cmkbr2 ELISA Kit, mJe-r ELISA Kit, C-C motif chemokine receptor 2 ELISA Kit, C-C motif chemokine receptor like 2 ELISA Kit, chemokine (C-C motif) receptor 2 ELISA Kit, CCR2 ELISA Kit, CCRL2 ELISA Kit, Ccr2 ELISA Kit, LOC484790 ELISA Kit

Background:

Gene Name: Chemokine C-C-Motif Receptor 2

Gene Aliases: CD192, CC-CKR2, CCR2A, C-C CKR-2, CCR2B, CKR2-A, CKR2-B, CMKBR2, MCP1-R, Monocyte chemoattractant protein 1 receptor

Pathways: cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process