CERK ELISA Kit

Catalog No. ABIN5652767

Product Details CERK ELISA Kit

Target: CERK (Ceramide Kinase (CERK))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12 ng/mL - 200 ng/mL

Minimum Detection Limit: 3.12 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ceramide Kinase (CERK). No significant cross-reactivity or interference between Ceramide Kinase (CERK) and analogues was observed.

Sensitivity: 1.14 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ceramide Kinase (CERK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ceramide Kinase (CERK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ceramide Kinase (CERK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ceramide Kinase (CERK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ceramide Kinase (CERK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ceramide Kinase (CERK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CERK

Target: CERK (Ceramide Kinase (CERK))

Alternative Name: Ceramide Kinase (CERK Products)

Synonyms: AI848610 ELISA Kit, D330016D08Rik ELISA Kit, mKIAA1646 ELISA Kit, LK4 ELISA Kit, dA59H18.2 ELISA Kit, dA59H18.3 ELISA Kit, hCERK ELISA Kit, CERK ELISA Kit, si:ch211-162i19.1 ELISA Kit, MGC81777 ELISA Kit, CG16708 ELISA Kit, DCERK ELISA Kit, Dcerk ELISA Kit, Dmel\\CG16708 ELISA Kit, dcerk ELISA Kit, ceramide kinase ELISA Kit, ceramide kinase S homeolog ELISA Kit, Ceramide kinase ELISA Kit, Cerk ELISA Kit, CERK ELISA Kit, cerk ELISA Kit, cerk.S ELISA Kit, CpipJ_CPIJ003342 ELISA Kit, LOAG_11245 ELISA Kit, LOC5579301 ELISA Kit, Bm1_50005 ELISA Kit

Background:

Gene Name: Ceramide Kinase

Gene Aliases: LK4, hCERK, Lipid kinase 4, Acylsphingosine kinase