CADM1 ELISA Kit

Catalog No. ABIN5652756

Product Details CADM1 ELISA Kit

Target: CADM1 (Cell Adhesion Molecule 1 (CADM1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cell Adhesion Molecule 1 (CADM1). No significant cross-reactivity or interference between Cell Adhesion Molecule 1 (CADM1) and analogues was observed.

Sensitivity: 0.071 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cell Adhesion Molecule 1 (CADM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cell Adhesion Molecule 1 (CADM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cell Adhesion Molecule 1 (CADM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cell Adhesion Molecule 1 (CADM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cell Adhesion Molecule 1 (CADM1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cell Adhesion Molecule 1 (CADM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CADM1

Target: CADM1 (Cell Adhesion Molecule 1 (CADM1))

Alternative Name: Cell Adhesion Molecule 1 (CADM1 Products)

Synonyms: BL2 ELISA Kit, IGSF4 ELISA Kit, IGSF4A ELISA Kit, NECL2 ELISA Kit, Necl-2 ELISA Kit, RA175 ELISA Kit, ST17 ELISA Kit, SYNCAM ELISA Kit, TSLC1 ELISA Kit, sTSLC-1 ELISA Kit, sgIGSF ELISA Kit, synCAM1 ELISA Kit, bl2 ELISA Kit, igsf4 ELISA Kit, igsf4a ELISA Kit, necl-2 ELISA Kit, necl2 ELISA Kit, ra175 ELISA Kit, sgigsf ELISA Kit, st17 ELISA Kit, stslc-1 ELISA Kit, syncam ELISA Kit, syncam1 ELISA Kit, tslc1 ELISA Kit, xnecl2 ELISA Kit, 2900073G06Rik ELISA Kit, 3100001I08Rik ELISA Kit, AI987920 ELISA Kit, Bl2 ELISA Kit, Igsf4 ELISA Kit, Igsf4a ELISA Kit, Necl2 ELISA Kit, RA175A ELISA Kit, RA175B ELISA Kit, RA175C ELISA Kit, RA175N ELISA Kit, SgIGSF ELISA Kit, SynCam ELISA Kit, Tslc1 ELISA Kit, SynCAM1 ELISA Kit, cell adhesion molecule 1 ELISA Kit, cell adhesion molecule 1 L homeolog ELISA Kit, CADM1 ELISA Kit, cadm1.L ELISA Kit, Cadm1 ELISA Kit

Background:

Gene Name: Cell Adhesion Molecule 1

Gene Aliases: BL2, IGSF4, IGSF4A, NECL2, RA175, ST17, SYNCAM, TSLC1, sTSLC-1, synCAM1, Tumor Suppressor In Lung Cancer 1, Immunoglobulin Superfamily,Member 4, Nectin-Like 2

Gene ID: 23705

UniProt: Q9BY67

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Activated T Cell Proliferation