CTNNA1 ELISA Kit

Catalog No. ABIN5652678

Product Details CTNNA1 ELISA Kit

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Catenin Alpha 1 (CTNNa1). No significant cross-reactivity or interference between Catenin Alpha 1 (CTNNa1) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Catenin Alpha 1 (CTNNa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Catenin Alpha 1 (CTNNa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Catenin Alpha 1 (CTNNa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Catenin Alpha 1 (CTNNa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Catenin Alpha 1 (CTNNa1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Catenin Alpha 1 (CTNNa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CTNNA1

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Alternative Name: Catenin Alpha 1 (CTNNA1 Products)

Synonyms: CAP102 ELISA Kit, 2010010M04Rik ELISA Kit, AA517462 ELISA Kit, AI988031 ELISA Kit, Catna1 ELISA Kit, CG17947 ELISA Kit, CT39986 ELISA Kit, Dalpha-Cat ELISA Kit, Dalpha-cat ELISA Kit, DalphaC ELISA Kit, Dalphacat ELISA Kit, Dmcatp ELISA Kit, Dmel\\CG17947 ELISA Kit, a-Cat ELISA Kit, a-cat ELISA Kit, alpha-CAT ELISA Kit, alpha-cat ELISA Kit, alpha-catenin ELISA Kit, alpha-ctn ELISA Kit, alphaCAT ELISA Kit, alphaCat ELISA Kit, alphacat ELISA Kit, Alpha-Cat ELISA Kit, dgri_GLEANR_3829 ELISA Kit, DgriGH19548 ELISA Kit, GH19548 ELISA Kit, ctnna ELISA Kit, cap102 ELISA Kit, catna1 ELISA Kit, ctnna1 ELISA Kit, catenin alpha 1 ELISA Kit, catenin (cadherin associated protein), alpha 1 ELISA Kit, alpha Catenin ELISA Kit, catenin alpha-1 ELISA Kit, catenin (cadherin-associated protein), alpha 1 ELISA Kit, catenin alpha 1 S homeolog ELISA Kit, CTNNA1 ELISA Kit, Ctnna1 ELISA Kit, alpha-Cat ELISA Kit, Dgri\alpha-Cat ELISA Kit, Tsp_10127 ELISA Kit, ctnna1 ELISA Kit, ctnna1.S ELISA Kit

Background:

Gene Name: Catenin Alpha 1

Gene Aliases: CTNN-A1, aCat, CAP102, Cadherin Associated Protein Alpha 1, Alpha E-catenin, Renal carcinoma antigen NY-REN-13

Gene ID: 1495

UniProt: P35221

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location