Casein Kinase 1 delta ELISA Kit

Catalog No. ABIN5652639

Product Details Casein Kinase 1 delta ELISA Kit

Target: Casein Kinase 1 delta (CSNK1D) (Casein Kinase 1, delta (CSNK1D))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Casein Kinase 1 Delta (CSNK1d). No significant cross-reactivity or interference between Casein Kinase 1 Delta (CSNK1d) and analogues was observed.

Sensitivity: 0.052 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Casein Kinase 1 Delta (CSNK1d). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Casein Kinase 1 Delta (CSNK1d). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Casein Kinase 1 Delta (CSNK1d), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Casein Kinase 1 Delta (CSNK1d) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Casein Kinase 1 Delta (CSNK1d) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Casein Kinase 1 Delta (CSNK1d) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Casein Kinase 1 delta

Target: Casein Kinase 1 delta (CSNK1D) (Casein Kinase 1, delta (CSNK1D))

Alternative Name: Casein Kinase 1 Delta (CSNK1D Products)

Synonyms: ASPS ELISA Kit, CKIdelta ELISA Kit, FASPS2 ELISA Kit, HCKID ELISA Kit, 1200006A05Rik ELISA Kit, AA409348 ELISA Kit, D930010H05Rik ELISA Kit, CKI-delta ELISA Kit, CKId ELISA Kit, csnk1d ELISA Kit, wu:fb69b03 ELISA Kit, wu:fj97a05 ELISA Kit, zgc:77627 ELISA Kit, CSNK1D ELISA Kit, ckIdelta ELISA Kit, Csnk1d ELISA Kit, csnk1dl ELISA Kit, wu:fb50c04 ELISA Kit, zgc:63971 ELISA Kit, casein kinase 1 delta ELISA Kit, casein kinase 1, delta ELISA Kit, casein kinase 1, delta b ELISA Kit, casein kinase 1 delta S homeolog ELISA Kit, casein kinase 1, delta a ELISA Kit, CSNK1D ELISA Kit, Csnk1d ELISA Kit, csnk1d ELISA Kit, csnk1db ELISA Kit, Bm1_37175 ELISA Kit, csnk1d.S ELISA Kit, csnk1da ELISA Kit

Background:

Gene Name: Casein Kinase 1 Delta

Gene Aliases: HCKID, Tau-protein kinase CSNK1D, Casein kinase I isoform delta

Gene ID: 1453

UniProt: P48730

Pathways: Hedgehog Signaling, M Phase