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CEACAM8 ELISA Kit

CEACAM8 Reactivity: Human Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5652593
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    CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.62 pg/mL - 1000 pg/mL
    Minimum Detection Limit
    15.62 pg/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8). No significant cross-reactivity or interference between Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) and analogues was observed.
    Sensitivity
    5.8 pg/mL
    Featured
    Discover our best selling CEACAM8 ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all CEACAM8 products
    CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))
    Alternative Name
    Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8 Products)
    Synonyms
    CD67 ELISA Kit, CGM6 ELISA Kit, CD66b ELISA Kit, NCA-95 ELISA Kit, CEACAM8 ELISA Kit, CEACAM1 ELISA Kit, MGC142545 ELISA Kit, cd67 ELISA Kit, cgm6 ELISA Kit, cd66b ELISA Kit, nca-95 ELISA Kit, carcinoembryonic antigen related cell adhesion molecule 8 ELISA Kit, carcinoembryonic antigen related cell adhesion molecule 1 ELISA Kit, carcinoembryonic antigen-related cell adhesion molecule 8 ELISA Kit, CEACAM8 ELISA Kit, CEACAM1 ELISA Kit, LOC612469 ELISA Kit, ceacam8 ELISA Kit
    Background

    Gene Name: Carcinoembryonic Antigen Related Cell Adhesion Molecule 8

    Gene Aliases: CD67, CD66-B, CD66b, CGM6, NCA95, Carcinoembryonic antigen CGM6, Non-specific cross-reacting antigen NCA-95

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