CPN1 ELISA Kit

Catalog No. ABIN5652580

Product Details CPN1 ELISA Kit

Target: CPN1 (Carboxypeptidase N Subunit 1 (CPN1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Carboxypeptidase N1 (CPN1). No significant cross-reactivity or interference between Carboxypeptidase N1 (CPN1) and analogues was observed.

Sensitivity: 24.2 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase N1 (CPN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase N1 (CPN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase N1 (CPN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carboxypeptidase N1 (CPN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carboxypeptidase N1 (CPN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carboxypeptidase N1 (CPN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CPN1

Target: CPN1 (Carboxypeptidase N Subunit 1 (CPN1))

Alternative Name: Carboxypeptidase N1 (CPN1 Products)

Synonyms: CPN ELISA Kit, SCPN ELISA Kit, Cpn ELISA Kit, cb1037 ELISA Kit, fa99g08 ELISA Kit, wu:fa99g08 ELISA Kit, zgc:77485 ELISA Kit, cpn ELISA Kit, scpn ELISA Kit, 0610011F20Rik ELISA Kit, carboxypeptidase N subunit 1 ELISA Kit, carboxypeptidase N, polypeptide 1 ELISA Kit, carboxypeptidase N subunit 1 S homeolog ELISA Kit, CPN1 ELISA Kit, Cpn1 ELISA Kit, cpn1 ELISA Kit, cpn1.S ELISA Kit

Background:

Gene Name: Carboxypeptidase N1

Gene Aliases: CPN, SCPN, ACBP, Anaphylatoxin inactivator, Arginine carboxypeptidase, Kininase-1, Lysine carboxypeptidase, Plasma carboxypeptidase B, Serum carboxypeptidase N

Gene ID: 93721

UniProt: Q9JJN5

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, C21-Steroid Hormone Metabolic Process