Calpain S1 ELISA Kit

Catalog No. ABIN5652517

Product Details Calpain S1 ELISA Kit

Target: Calpain S1 (CAPNS1) (Calpain, Small Subunit 1 (CAPNS1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Calpain, Small Subunit 1 (CAPNS1). No significant cross-reactivity or interference between Calpain, Small Subunit 1 (CAPNS1) and analogues was observed.

Sensitivity: 0.126 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpain, Small Subunit 1 (CAPNS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calpain, Small Subunit 1 (CAPNS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calpain, Small Subunit 1 (CAPNS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calpain, Small Subunit 1 (CAPNS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calpain, Small Subunit 1 (CAPNS1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calpain, Small Subunit 1 (CAPNS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Calpain S1

Target: Calpain S1 (CAPNS1) (Calpain, Small Subunit 1 (CAPNS1))

Alternative Name: Calpain, Small Subunit 1 (CAPNS1 Products)

Synonyms: 30K ELISA Kit, CALPAIN4 ELISA Kit, CANP ELISA Kit, CANPS ELISA Kit, CAPN4 ELISA Kit, CDPS ELISA Kit, CSS1 ELISA Kit, Capn4 ELISA Kit, CAPNS1 ELISA Kit, Capa-4 ELISA Kit, Capa4 ELISA Kit, D7Ertd146e ELISA Kit, MGC84330 ELISA Kit, capns1 ELISA Kit, cb616 ELISA Kit, wu:fb81g08 ELISA Kit, wu:fl09e04 ELISA Kit, zgc:110603 ELISA Kit, calpain small subunit 1 ELISA Kit, calpain, small subunit 1 ELISA Kit, calpain, small subunit 1 L homeolog ELISA Kit, calpain, small subunit 1 a ELISA Kit, CAPNS1 ELISA Kit, Capns1 ELISA Kit, capns1.L ELISA Kit, capns1a ELISA Kit

Background:

Gene Name: Calpain, Small Subunit 1

Gene Aliases: CANP, 30K, Calpain 4, CANPS, CAPN4, CDPS, Calcium-activated neutral proteinase small subunit, Calcium-dependent protease small subunit, Calpain regulatory subunit

Gene ID: 826

UniProt: P04632

Pathways: Apoptosis