CAMK2B ELISA Kit

Catalog No. ABIN5652498

Product Details CAMK2B ELISA Kit

Target: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b). No significant cross-reactivity or interference between Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) and analogues was observed.

Sensitivity: 0.113 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CAMK2B

Target: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Alternative Name: Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2B Products)

Synonyms: CAM2 ELISA Kit, CAMK2 ELISA Kit, CAMKB ELISA Kit, Camk2d ELISA Kit, Ck2b ELISA Kit, camk2 ELISA Kit, camk2b ELISA Kit, cam2 ELISA Kit, camkb ELISA Kit, CAMK2B ELISA Kit, calcium/calmodulin dependent protein kinase II beta ELISA Kit, calcium/calmodulin-dependent protein kinase II, beta ELISA Kit, calcium/calmodulin-dependent protein kinase II beta ELISA Kit, calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog ELISA Kit, calcium/calmodulin dependent protein kinase (CaM kinase) II beta L homeolog ELISA Kit, calcium/calmodulin-dependent protein kinase (CaM kinase) II beta ELISA Kit, CAMK2B ELISA Kit, Camk2b ELISA Kit, camk2a.S ELISA Kit, camk2b.L ELISA Kit

Background:

Gene Name: Calcium/Calmodulin Dependent Protein Kinase II Beta

Gene Aliases: CAM2, CAMK2, CAMKB, Proline Rich Calmodulin-Dependent Protein Kinase

Gene ID: 816

UniProt: Q13554

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity