CAMK2A ELISA Kit

Catalog No. ABIN5652497

Product Details CAMK2A ELISA Kit

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a). No significant cross-reactivity or interference between Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Alternative Name: Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2A Products)

Synonyms: camk2 ELISA Kit, camk2b ELISA Kit, zgc:112538 ELISA Kit, zgc:123320 ELISA Kit, CAMKA ELISA Kit, CaMKII ELISA Kit, R74975 ELISA Kit, mKIAA0968 ELISA Kit, PK2CDD ELISA Kit, PKCCD ELISA Kit, calcium/calmodulin dependent protein kinase II alpha ELISA Kit, calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog ELISA Kit, calcium/calmodulin-dependent protein kinase II alpha ELISA Kit, CAMK2A ELISA Kit, camk2a.S ELISA Kit, camk2a ELISA Kit, Camk2a ELISA Kit

Background:

Gene Name: Calcium/Calmodulin Dependent Protein Kinase II Alpha

Gene Aliases: CAMKA

Gene ID: 25400

UniProt: P11275

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction