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C-Peptide ELISA Kit

Reactivity: Dog Colorimetric Competition ELISA 98.77 pg/mL - 8000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5652402
  • Target See all C-Peptide ELISA Kits
    C-Peptide
    Reactivity
    • 8
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dog
    Detection Method
    Colorimetric
    Method Type
    Competition ELISA
    Detection Range
    98.77 pg/mL - 8000 pg/mL
    Minimum Detection Limit
    98.77 pg/mL
    Application
    ELISA
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of C-Peptide (CP). No significant cross-reactivity or interference between C-Peptide (CP) and analogues was observed.
    Sensitivity
    36.65 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 h
    Plate
    Pre-coated
    Protocol
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to C-Peptide (CP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled C-Peptide (CP) and unlabeled C-Peptide (CP) (Standards or samples) with the pre-coated antibody specific to C-Peptide (CP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of C-Peptide (CP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of C-Peptide (CP) in the sample.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Peptide (CP) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Peptide (CP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all C-Peptide ELISA Kits
    C-Peptide
    Abstract
    C-Peptide Products
    Synonyms
    insulin 2 ELISA Kit, Ins2 ELISA Kit
    Background

    Gene Name: C-Peptide

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