BMPR1A ELISA Kit

Catalog No. ABIN5652298

Product Details BMPR1A ELISA Kit

Target: BMPR1A (Bone Morphogenetic Protein Receptor 1A (BMPR1A))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein Receptor 1A (BMPR1A). No significant cross-reactivity or interference between Bone Morphogenetic Protein Receptor 1A (BMPR1A) and analogues was observed.

Sensitivity: 0.24 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein Receptor 1A (BMPR1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein Receptor 1A (BMPR1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein Receptor 1A (BMPR1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein Receptor 1A (BMPR1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 1A (BMPR1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 1A (BMPR1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for BMPR1A

Target: BMPR1A (Bone Morphogenetic Protein Receptor 1A (BMPR1A))

Alternative Name: Bone Morphogenetic Protein Receptor 1A (BMPR1A Products)

Synonyms: 10q23del ELISA Kit, ACVRLK3 ELISA Kit, ALK3 ELISA Kit, CD292 ELISA Kit, SKR5 ELISA Kit, 1110037I22Rik ELISA Kit, AU045487 ELISA Kit, BMPR-IA ELISA Kit, Bmpr ELISA Kit, BMPR-1 ELISA Kit, BRK-1 ELISA Kit, BMPR1A ELISA Kit, acvrlk3 ELISA Kit, alk-3 ELISA Kit, alk3 ELISA Kit, cd292 ELISA Kit, skr5 ELISA Kit, BR1a ELISA Kit, alk3tr ELISA Kit, bmpr1a ELISA Kit, zgc:136731 ELISA Kit, wu:fe05b06 ELISA Kit, wu:fe06e10 ELISA Kit, zgc:92227 ELISA Kit, bone morphogenetic protein receptor type 1A ELISA Kit, bone morphogenetic protein receptor, type 1A ELISA Kit, bone morphogenetic protein receptor type 1A S homeolog ELISA Kit, bone morphogenetic protein receptor, type IAa ELISA Kit, bone morphogenetic protein receptor, type IAb ELISA Kit, BMPR1A ELISA Kit, Bmpr1a ELISA Kit, bmpr1a ELISA Kit, bmpr1a.S ELISA Kit, bmpr1aa ELISA Kit, bmpr1ab ELISA Kit

Background:

Gene Name: Bone Morphogenetic Protein Receptor 1A

Gene Aliases: CD292, ACVRLK3, ALK3, SKR5, BMPR1-A, Activin A Receptor, Type Ii-Like Kinase 3, Activin Receptor-Like Kinase 3, Serine/Threonine-Protein Kinase Receptor R5

Gene ID: 81507

UniProt: Q78EA7

Pathways: Stem Cell Maintenance