BMPER ELISA Kit

Catalog No. ABIN5652238

Product Details BMPER ELISA Kit

Target: BMPER (BMP Binding Endothelial Regulator (BMPER))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of BMP Binding Endothelial Regulator (BMPER). No significant cross-reactivity or interference between BMP Binding Endothelial Regulator (BMPER) and analogues was observed.

Sensitivity: 0.058 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BMP Binding Endothelial Regulator (BMPER). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to BMP Binding Endothelial Regulator (BMPER). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BMP Binding Endothelial Regulator (BMPER), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BMP Binding Endothelial Regulator (BMPER) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BMP Binding Endothelial Regulator (BMPER) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BMP Binding Endothelial Regulator (BMPER) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for BMPER

Target: BMPER (BMP Binding Endothelial Regulator (BMPER))

Alternative Name: BMP Binding Endothelial Regulator (BMPER Products)

Synonyms: BMPER ELISA Kit, CG15671 ELISA Kit, CT35855 ELISA Kit, Cv 2 ELISA Kit, Cv-2 ELISA Kit, Cv2 ELISA Kit, Dmel\\CG15671 ELISA Kit, cv 2 ELISA Kit, CV2 ELISA Kit, cv-2 ELISA Kit, crim3 ELISA Kit, cv2 ELISA Kit, cvl2 ELISA Kit, id:ibd5071 ELISA Kit, CV-2 ELISA Kit, CRIM3 ELISA Kit, 3110056H04Rik ELISA Kit, Crim3 ELISA Kit, RGD1563373 ELISA Kit, BMP binding endothelial regulator ELISA Kit, crossveinless 2 ELISA Kit, BMP-binding endothelial regulator protein ELISA Kit, BMP binding endothelial regulator L homeolog ELISA Kit, BMP-binding endothelial regulator ELISA Kit, BMPER ELISA Kit, cv-2 ELISA Kit, bmper ELISA Kit, LOC100070275 ELISA Kit, cv2 ELISA Kit, bmper.L ELISA Kit, Bmper ELISA Kit

Background:

Gene Name: BMP Binding Endothelial Regulator

Gene Aliases: Cv2, CRIM3, Crossveinless-2, Bone morphogenetic protein-binding endothelial cell precursor-derived regulator