beta Endorphin CLIA Kit
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- Target See all beta Endorphin (beta-EP) CLIA Kits
- beta Endorphin (beta-EP) (Endorphin beta (beta-EP))
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Reactivity
- Mouse
- Detection Method
- Chemiluminescent
- Method Type
- Competition ELISA
- Detection Range
- 7.81 pg/mL - 2000 pg/mL
- Minimum Detection Limit
- 7.81 pg/mL
- Application
- ELISA
- Sample Type
- Cerebrospinal Fluid, Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Beta-Endorphin (bEP). No significant cross-reactivity or interference between Beta-Endorphin (bEP) and analogues was observed.
- Sensitivity
- 2.95 pg/mL
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- Discover our best selling beta-EP ELISA Kit
- Top Product
- Discover our top product beta-EP ELISA Kit
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 2 h
- Plate
- Pre-coated
- Protocol
- The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Beta-Endorphin (bEP). A competitive inhibition reaction is launched between biotin labeled Beta-Endorphin (bEP) and unlabeled Beta-Endorphin (bEP) (Standards or samples) with the pre-coated antibody specific to Beta-Endorphin (bEP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Beta-Endorphin (bEP) level in the sample or standard.
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all beta Endorphin (beta-EP) CLIA Kits
- beta Endorphin (beta-EP) (Endorphin beta (beta-EP))
- Alternative Name
- Beta-Endorphin (beta-EP Products)
- Synonyms
- ACTH CLIA Kit, BE CLIA Kit, Beta-LPH CLIA Kit, Clip CLIA Kit, Gamma-LPH CLIA Kit, Npp CLIA Kit, Pomc-1 CLIA Kit, Pomc1 CLIA Kit, alpha-MSH CLIA Kit, alphaMSH CLIA Kit, beta-MSH CLIA Kit, gamma-MSH CLIA Kit, CLIP CLIA Kit, LPH CLIA Kit, MSH CLIA Kit, NPP CLIA Kit, POC CLIA Kit, pro-opiomelanocortin-alpha CLIA Kit, proopiomelanocortin CLIA Kit, Pomc CLIA Kit, POMC CLIA Kit
- Background
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Gene Name: Beta-Endorphin
Gene Aliases: b-EP
- Pathways
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
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