BNIP3 CLIA Kit

Catalog No. ABIN5652170

Product Details BNIP3 CLIA Kit

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Reactivity: Rat

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3 (BNIP3). No significant cross-reactivity or interference between Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3 (BNIP3) and analogues was observed.

Sensitivity: 0.03 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3 (BNIP3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3 (BNIP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3 (BNIP3) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Alternative Name: Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3 Products)

Synonyms: NIP3 CLIA Kit, Nip3 CLIA Kit, BCL2 interacting protein 3 CLIA Kit, BCL2/adenovirus E1B interacting protein 3 CLIA Kit, BNIP3 CLIA Kit, Bnip3 CLIA Kit

Background:

Gene Name: Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3

Gene Aliases: NIP3

Gene ID: 84480

UniProt: Q9ET45

Pathways: Autophagy, Brown Fat Cell Differentiation