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BCL2L1 ELISA Kit

BCL2L1 Reactivity: Mouse Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN5652134
  • Target See all BCL2L1 ELISA Kits
    BCL2L1 (BCL2-Like 1 (BCL2L1))
    Reactivity
    • 6
    • 4
    • 3
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    7.81 pg/mL - 500 pg/mL
    Minimum Detection Limit
    7.81 pg/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L). No significant cross-reactivity or interference between B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) and analogues was observed.
    Sensitivity
    3.1 pg/mL
    Top Product
    Discover our top product BCL2L1 ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all BCL2L1 ELISA Kits
    BCL2L1 (BCL2-Like 1 (BCL2L1))
    Alternative Name
    B-Cell CLL/Lymphoma 2 Like Protein (BCL2L1 Products)
    Synonyms
    BCL-XL/S ELISA Kit, BCL2L ELISA Kit, BCLX ELISA Kit, BCLXL ELISA Kit, BCLXS ELISA Kit, Bcl-X ELISA Kit, PPP1R52 ELISA Kit, bcl-xL ELISA Kit, bcl-xS ELISA Kit, Bcl(X)L ELISA Kit, Bcl-XL ELISA Kit, Bcl-Xs ELISA Kit, Bcl2l ELISA Kit, BclX ELISA Kit, bcl-x ELISA Kit, Bcl-xl ELISA Kit, Bclx ELISA Kit, bcl-X ELISA Kit, Bcl-xL ELISA Kit, BCL-XL ELISA Kit, BCL2L1 ELISA Kit, bcl-XL ELISA Kit, bcl-x(l) ELISA Kit, bcl-xl/s ELISA Kit, bcl-xs ELISA Kit, bcl2l ELISA Kit, bclx ELISA Kit, bclxl ELISA Kit, xR11 ELISA Kit, bcl-xl ELISA Kit, blp1 ELISA Kit, cb1021 ELISA Kit, zfBLP1 ELISA Kit, BCL2 like 1 ELISA Kit, BCL2-like 1 ELISA Kit, Bcl2-like 1 ELISA Kit, BCL2-like 1 L homeolog ELISA Kit, bcl2-like 1 ELISA Kit, BCL2L1 ELISA Kit, Bcl2l1 ELISA Kit, bcl2l1 ELISA Kit, bcl2l1.L ELISA Kit
    Background

    Gene Name: B-Cell CLL/Lymphoma 2 Like Protein

    Gene Aliases: BCL-XL/S, BCL2L1, BCLX, Bcl-X, bcl-xL, bcl-xS, Apoptosis regulator Bcl-X

    Gene ID
    12048
    UniProt
    Q64373
    Pathways
    Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
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