ATG7 ELISA Kit

Catalog No. ABIN5652122

Product Details ATG7 ELISA Kit

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Autophagy Related Protein 7 (ATG7). No significant cross-reactivity or interference between Autophagy Related Protein 7 (ATG7) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy Related Protein 7 (ATG7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Autophagy Related Protein 7 (ATG7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autophagy Related Protein 7 (ATG7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Autophagy Related Protein 7 (ATG7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Autophagy Related Protein 7 (ATG7) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Autophagy Related Protein 7 (ATG7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: Autophagy Related Protein 7 (ATG7 Products)

Synonyms: APG7-LIKE ELISA Kit, APG7L ELISA Kit, GSA7 ELISA Kit, D8 ELISA Kit, UBA1B ELISA Kit, UBE1L ELISA Kit, UBE2 ELISA Kit, Ube1l ELISA Kit, 1300004C08Rik ELISA Kit, AO090001000744 ELISA Kit, atg7 ELISA Kit, 1810013K23Rik ELISA Kit, Agp7 ELISA Kit, Apg7l ELISA Kit, Atg7l ELISA Kit, ATAPG7 ELISA Kit, ATATG7 ELISA Kit, ATG7 ELISA Kit, AUTOPHAGY 7 ELISA Kit, AUTOPHAGY-RELATED 7 ELISA Kit, K15I22.10 ELISA Kit, K15I22_10 ELISA Kit, autophagy related 7 ELISA Kit, ubiquitin like modifier activating enzyme 7 ELISA Kit, ubiquitin-like modifier activating enzyme 7 ELISA Kit, autophagy-related protein 7 ELISA Kit, Autophagy-related protein 7 ELISA Kit, Apg7p ELISA Kit, ThiF family protein ELISA Kit, ubiquitin-like conjugating enzyme Atg7 ELISA Kit, ATG7 ELISA Kit, UBA7 ELISA Kit, Uba7 ELISA Kit, ANI_1_3368024 ELISA Kit, AOR_1_1338164 ELISA Kit, PTRG_02236 ELISA Kit, PAAG_00287 ELISA Kit, PITG_12537 ELISA Kit, atg7 ELISA Kit, Atg7 ELISA Kit, APG7 ELISA Kit

Background:

Gene Name: Autophagy Related Protein 7

Gene Aliases: APG7L, GSA7, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Ubiquitin-activating enzyme E1-like protein

Gene ID: 74244

UniProt: Q9D906

Pathways: Response to Water Deprivation, Autophagy