ATG5 ELISA Kit

Catalog No. ABIN5652120

Product Details ATG5 ELISA Kit

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Autophagy Related Protein 5 (ATG5). No significant cross-reactivity or interference between Autophagy Related Protein 5 (ATG5) and analogues was observed.

Sensitivity: 0.28 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy Related Protein 5 (ATG5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Autophagy Related Protein 5 (ATG5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autophagy Related Protein 5 (ATG5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Autophagy Related Protein 5 (ATG5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Autophagy Related Protein 5 (ATG5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Autophagy Related Protein 5 (ATG5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: Autophagy Related Protein 5 (ATG5 Products)

Synonyms: APG5 ELISA Kit, APG5-LIKE ELISA Kit, APG5L ELISA Kit, ASP ELISA Kit, hAPG5 ELISA Kit, ATG5 ELISA Kit, CG1643 ELISA Kit, DmAtg5 ELISA Kit, Dmel\\CG1643 ELISA Kit, atg5 ELISA Kit, 2010107M05Rik ELISA Kit, 3110067M24Rik ELISA Kit, AW319544 ELISA Kit, Apg5l ELISA Kit, Atg5l ELISA Kit, C88337 ELISA Kit, Paddy ELISA Kit, apg5l ELISA Kit, zgc:100934 ELISA Kit, ATATG5 ELISA Kit, AUTOPHAGY 5 ELISA Kit, MKP11.20 ELISA Kit, MKP11_20 ELISA Kit, autophagy related 5 ELISA Kit, Autophagy-related 5 ELISA Kit, ATG5 autophagy related 5 homolog (S. cerevisiae) ELISA Kit, autophagy related 5 L homeolog ELISA Kit, similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12 ELISA Kit, autophagy protein Apg5 family ELISA Kit, ATG5 ELISA Kit, Atg5 ELISA Kit, atg5 ELISA Kit, atg5.L ELISA Kit, APG5 ELISA Kit

Background:

Gene Name: Autophagy Related Protein 5

Gene Aliases: ASP, APG5, APG5L, hAPG5, Apoptosis-specific protein, Autophagy protein 5

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy