ATP1A1 ELISA Kit

Catalog No. ABIN5652094

Product Details ATP1A1 ELISA Kit

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reactivity: Human, Rat, Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). No significant cross-reactivity or interference between ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ATP1A1

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Alternative Name: ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1A1 Products)

Synonyms: Atpa-1 ELISA Kit, BC010319 ELISA Kit, Nkaa1b ELISA Kit, K-ATPase ELISA Kit, Na+K+ATPase ELISA Kit, atp1a1 ELISA Kit, atp1a1a.1 ELISA Kit, atp1a1l1 ELISA Kit, atp[a]1B1 ELISA Kit, cb70 ELISA Kit, wu:fa03c03 ELISA Kit, wu:fb92h10 ELISA Kit, atp1a1l5 ELISA Kit, atp[a]1B2 ELISA Kit, cb707 ELISA Kit, wu:fj34g01 ELISA Kit, zgc:92351 ELISA Kit, ATPase Na+/K+ transporting subunit alpha 1 ELISA Kit, ATPase, Na+/K+ transporting, alpha 1 polypeptide ELISA Kit, ATPase Na+/K+ transporting subunit alpha 1 L homeolog ELISA Kit, ATPase, Na+/K+ transporting, alpha 1a polypeptide, tandem duplicate 1 ELISA Kit, ATPase, Na+/K+ transporting, alpha 1b polypeptide ELISA Kit, ATP1A1 ELISA Kit, Atp1a1 ELISA Kit, atp1a1.L ELISA Kit, atp1a1a.1 ELISA Kit, atp1a1b ELISA Kit

Background:

Gene Name: ATPase, Na+/K+ Transporting Alpha 1 Polypeptide

Gene Aliases: Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium/potassium-transporting ATPase subunit alpha-1

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process