ABCB5 ELISA Kit

Catalog No. ABIN5652065

Product Details ABCB5 ELISA Kit

Target: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of ATP Binding Cassette Transporter B5 (ABCB5). No significant cross-reactivity or interference between ATP Binding Cassette Transporter B5 (ABCB5) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter B5 (ABCB5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter B5 (ABCB5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter B5 (ABCB5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP Binding Cassette Transporter B5 (ABCB5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter B5 (ABCB5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter B5 (ABCB5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ABCB5

Target: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Alternative Name: ATP Binding Cassette Transporter B5 (ABCB5 Products)

Synonyms: ABCB5alpha ELISA Kit, ABCB5beta ELISA Kit, EST422562 ELISA Kit, 9230106F14Rik ELISA Kit, RGD1566342 ELISA Kit, ABCB5 ELISA Kit, mdr1 ELISA Kit, im:7158730 ELISA Kit, wu:fc18f02 ELISA Kit, wu:fi81f06 ELISA Kit, ATP binding cassette subfamily B member 5 ELISA Kit, ATP-binding cassette, sub-family B (MDR/TAP), member 5 ELISA Kit, ATP-binding cassette sub-family B member 5 ELISA Kit, ABCB5 ELISA Kit, Abcb5 ELISA Kit, abcb5 ELISA Kit, TGME49_249820 ELISA Kit, PAAG_04387 ELISA Kit, VDBG_00160 ELISA Kit, VDBG_02292 ELISA Kit

Background:

Gene Name: ATP Binding Cassette Transporter B5

Gene Aliases: ABC-B5, P-Glycoprotein ABCB5, ATP-Binding Cassette Protein

Gene ID: 340273

UniProt: Q2M3G0