Aspartate beta Hydroxylase ELISA Kit

Catalog No. ABIN5652048

Product Details Aspartate beta Hydroxylase ELISA Kit

Target: Aspartate beta Hydroxylase (ASPH) (Aspartate beta-Hydroxylase (ASPH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Aspartate Beta Hydroxylase (ASPH). No significant cross-reactivity or interference between Aspartate Beta Hydroxylase (ASPH) and analogues was observed.

Sensitivity: 0.057 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aspartate Beta Hydroxylase (ASPH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aspartate Beta Hydroxylase (ASPH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aspartate Beta Hydroxylase (ASPH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aspartate Beta Hydroxylase (ASPH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aspartate Beta Hydroxylase (ASPH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aspartate Beta Hydroxylase (ASPH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Aspartate beta Hydroxylase

Target: Aspartate beta Hydroxylase (ASPH) (Aspartate beta-Hydroxylase (ASPH))

Alternative Name: Aspartate Beta Hydroxylase (ASPH Products)

Synonyms: AAH ELISA Kit, BAH ELISA Kit, CASQ2BP1 ELISA Kit, HAAH ELISA Kit, JCTN ELISA Kit, junctin ELISA Kit, 2310005F16Rik ELISA Kit, 3110001L23Rik ELISA Kit, AI848629 ELISA Kit, AW261690 ELISA Kit, AW561901 ELISA Kit, C79816 ELISA Kit, cI-37 ELISA Kit, aah ELISA Kit, bah ELISA Kit, jctn ELISA Kit, MGC107896 ELISA Kit, cb971 ELISA Kit, fb69e10 ELISA Kit, fc06d04 ELISA Kit, fc95a08 ELISA Kit, wu:fb69e10 ELISA Kit, wu:fc06d04 ELISA Kit, wu:fc95a08 ELISA Kit, aspartate beta-hydroxylase ELISA Kit, aspartate-beta-hydroxylase ELISA Kit, aspartate beta-hydroxylase L homeolog ELISA Kit, ASPH ELISA Kit, Asph ELISA Kit, asph ELISA Kit, asph.L ELISA Kit

Background:

Gene Name: Aspartate Beta Hydroxylase

Gene Aliases: BAH, CASQ2BP1, HAAH, JCTN, Junctin, Humbug, Junctate, Aspartyl/Asparaginyl Beta-Hydroxylase, Peptide-aspartate beta-dioxygenase

Gene ID: 444

UniProt: Q12797

Pathways: Positive Regulation of Endopeptidase Activity