GOT2 ELISA Kit

Catalog No. ABIN5652047

Product Details GOT2 ELISA Kit

Target: GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Aspartate Aminotransferase 2 (AST2). No significant cross-reactivity or interference between Aspartate Aminotransferase 2 (AST2) and analogues was observed.

Sensitivity: 0.29 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aspartate Aminotransferase 2 (AST2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aspartate Aminotransferase 2 (AST2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aspartate Aminotransferase 2 (AST2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aspartate Aminotransferase 2 (AST2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aspartate Aminotransferase 2 (AST2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aspartate Aminotransferase 2 (AST2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GOT2

Target: GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))

Alternative Name: Aspartate Aminotransferase 2 (GOT2 Products)

Synonyms: KAT4 ELISA Kit, KATIV ELISA Kit, mitAAT ELISA Kit, AI789014 ELISA Kit, Got-1 ELISA Kit, cAspAT ELISA Kit, cCAT ELISA Kit, AL022787 ELISA Kit, FABP-pm ELISA Kit, Got-2 ELISA Kit, mAspAT ELISA Kit, ASPATA ELISA Kit, got2 ELISA Kit, zgc:66329 ELISA Kit, fj40h07 ELISA Kit, wu:fj40h07 ELISA Kit, zgc:56425 ELISA Kit, glutamic-oxaloacetic transaminase 2 ELISA Kit, glutamic-oxaloacetic transaminase 1, soluble ELISA Kit, glutamatic-oxaloacetic transaminase 2, mitochondrial ELISA Kit, glutamic-oxaloacetic transaminase 2a, mitochondrial ELISA Kit, glutamic-oxaloacetic transaminase 2 L homeolog ELISA Kit, Aspartate amino transferase activity ELISA Kit, glutamic-oxaloacetic transaminase 2b, mitochondrial ELISA Kit, GOT2 ELISA Kit, got2 ELISA Kit, Got1 ELISA Kit, Got2 ELISA Kit, got2a ELISA Kit, got2.L ELISA Kit, AST ELISA Kit, got2b ELISA Kit

Background:

Gene Name: Aspartate Aminotransferase 2

Gene Aliases: GOT2, MitAAT, KATIV, KAT4, FABP-1, Plasma membrane-associated fatty acid-binding protein, Glutamic-Oxaloacetic Transaminase 2,Mitochondrial, Kynurenine Aminotransferase IV

Gene ID: 14719

UniProt: P05202

Pathways: Monocarboxylic Acid Catabolic Process