Asparagine Synthetase ELISA Kit

Catalog No. ABIN5652040

Product Details Asparagine Synthetase ELISA Kit

Target: Asparagine Synthetase (ASNS)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Asparagine Synthetase (ASNS). No significant cross-reactivity or interference between Asparagine Synthetase (ASNS) and analogues was observed.

Sensitivity: 0.28 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Asparagine Synthetase (ASNS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Asparagine Synthetase (ASNS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Asparagine Synthetase (ASNS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Asparagine Synthetase (ASNS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Asparagine Synthetase (ASNS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Asparagine Synthetase (ASNS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Asparagine Synthetase

Target: Asparagine Synthetase (ASNS)

Alternative Name: Asparagine Synthetase (ASNS Products)

Synonyms: zgc:56605 ELISA Kit, zgc:76901 ELISA Kit, LOC692995 ELISA Kit, ASNS ELISA Kit, GB13219 ELISA Kit, CG33486 ELISA Kit, Dmel\\CG33486 ELISA Kit, asns ELISA Kit, TS11 ELISA Kit, ARABIDOPSIS THALIANA GLUTAMINE-DEPENDENT ASPARAGINE SYNTHASE 1 ELISA Kit, AT-ASN1 ELISA Kit, DARK INDUCIBLE 6 ELISA Kit, DIN6 ELISA Kit, GLUTAMINE-DEPENDENT ASPARAGINE SYNTHETASE ELISA Kit, glutamine-dependent asparagine synthase 1 ELISA Kit, AS ELISA Kit, AsnS1 ELISA Kit, asparagine synthetase ELISA Kit, asparagine synthetase (glutamine-hydrolyzing) ELISA Kit, asparagine synthetase [glutamine-hydrolyzing] ELISA Kit, Asparagine synthetase ELISA Kit, glutamine-dependent asparagine synthase 1 ELISA Kit, asparagine synthetase homolog 1 ELISA Kit, asns ELISA Kit, ASNS ELISA Kit, LOC692995 ELISA Kit, LOC724480 ELISA Kit, asnB ELISA Kit, AsnS ELISA Kit, Asns ELISA Kit, ASN1 ELISA Kit, asn1 ELISA Kit

Background:

Gene Name: Asparagine Synthetase

Gene Aliases: Aspartate-Ammonia Ligase, Asparagine synthetase [glutamine-hydrolyzing], Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase

Gene ID: 440

UniProt: P08243

Pathways: ER-Nucleus Signaling