AFMID ELISA Kit

Catalog No. ABIN5652033

Product Details AFMID ELISA Kit

Target: AFMID (Arylformamidase (AFMID))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Arylformamidase (AFMID). No significant cross-reactivity or interference between Arylformamidase (AFMID) and analogues was observed.

Sensitivity: 0.57 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arylformamidase (AFMID). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arylformamidase (AFMID). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arylformamidase (AFMID), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arylformamidase (AFMID) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arylformamidase (AFMID) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arylformamidase (AFMID) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for AFMID

Target: AFMID (Arylformamidase (AFMID))

Alternative Name: Arylformamidase (AFMID Products)

Synonyms: FKF ELISA Kit, KF ELISA Kit, KFA ELISA Kit, 9030621K19Rik ELISA Kit, Ammd ELISA Kit, Kf ELISA Kit, formylase ELISA Kit, im:7148587 ELISA Kit, zgc:112472 ELISA Kit, arylformamidase ELISA Kit, Arylformamidase ELISA Kit, AFMID ELISA Kit, Afmid ELISA Kit, CNH01830 ELISA Kit, LACBIDRAFT_176573 ELISA Kit, EAT1b_1505 ELISA Kit, Alide_1381 ELISA Kit, Varpa_1604 ELISA Kit, Deima_1562 ELISA Kit, BC1001_2878 ELISA Kit, Deipr_2232 ELISA Kit, Acav_1168 ELISA Kit, Marky_1513 ELISA Kit, Alide2_3548 ELISA Kit, DelCs14_4522 ELISA Kit, afmid ELISA Kit

Background:

Gene Name: Arylformamidase

Gene Aliases: KF, KFA, FKF, Kynurenine formamidase, N-formylkynurenine formamidase