ALOX12 ELISA Kit

Catalog No. ABIN5651991

Product Details ALOX12 ELISA Kit

Target: ALOX12 (Arachidonate 12-Lipoxygenase (ALOX12))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Arachidonate-12-Lipoxygenase (ALOX12). No significant cross-reactivity or interference between Arachidonate-12-Lipoxygenase (ALOX12) and analogues was observed.

Sensitivity: 0.116 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-12-Lipoxygenase (ALOX12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate-12-Lipoxygenase (ALOX12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arachidonate-12-Lipoxygenase (ALOX12) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arachidonate-12-Lipoxygenase (ALOX12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ALOX12

Target: ALOX12 (Arachidonate 12-Lipoxygenase (ALOX12))

Alternative Name: Arachidonate-12-Lipoxygenase (ALOX12 Products)

Synonyms: 15-LOX-1 ELISA Kit, 15LOX-1 ELISA Kit, 12-LOX ELISA Kit, 12S-LOX ELISA Kit, LOG12 ELISA Kit, ALOX12 ELISA Kit, ALOX15 ELISA Kit, 9930022G08Rik ELISA Kit, Alox12p ELISA Kit, P-12LO ELISA Kit, 12-LO ELISA Kit, zgc:64120 ELISA Kit, wu:fb72a11 ELISA Kit, arachidonate 15-lipoxygenase ELISA Kit, arachidonate 12-lipoxygenase, 12S type ELISA Kit, arachidonate 12-lipoxygenase ELISA Kit, arachidonate 12-lipoxygenase, 12S-type ELISA Kit, ALOX15 ELISA Kit, ALOX12 ELISA Kit, Alox12 ELISA Kit, alox12 ELISA Kit, LOC100072916 ELISA Kit

Background:

Gene Name: Arachidonate-12-Lipoxygenase

Gene Aliases: LOX12, 12-LOX, ALOX-12, 12-LO, 12LPG, LOG12, Lipoxygenase 12, 12S-lipoxygenase, Lipoxin synthase 12-LO, Platelet-type lipoxygenase 12

Gene ID: 239

UniProt: P18054

Pathways: Positive Regulation of Endopeptidase Activity