Anillin ELISA Kit

Catalog No. ABIN5651818

Product Details Anillin ELISA Kit

Target: Anillin (ANLN) (Anillin, Actin Binding Protein (ANLN))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Anillin (ANLN). No significant cross-reactivity or interference between Anillin (ANLN) and analogues was observed.

Sensitivity: 11.5 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anillin (ANLN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Anillin (ANLN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anillin (ANLN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anillin (ANLN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anillin (ANLN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anillin (ANLN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Anillin

Target: Anillin (ANLN) (Anillin, Actin Binding Protein (ANLN))

Alternative Name: Anillin (ANLN Products)

Synonyms: Scraps ELISA Kit, scra ELISA Kit, ANLN ELISA Kit, 1110037A17Rik ELISA Kit, 2900037I21Rik ELISA Kit, C78101 ELISA Kit, scraps ELISA Kit, ABP8 ELISA Kit, Abp8 ELISA Kit, Ani ELISA Kit, Anil ELISA Kit, Anillin ELISA Kit, CG2092 ELISA Kit, Dmel\\CG2092 ELISA Kit, Scra ELISA Kit, ani ELISA Kit, anil ELISA Kit, anillin ELISA Kit, l(2)03427 ELISA Kit, l(2)43Ec ELISA Kit, l(2)k08255 ELISA Kit, RGD1561367 ELISA Kit, si:ch211-197n10.2 ELISA Kit, wu:fb74d05 ELISA Kit, wu:fd10d09 ELISA Kit, wu:fi41f04 ELISA Kit, anillin actin binding protein ELISA Kit, anillin, actin binding protein ELISA Kit, scraps ELISA Kit, anillin actin binding protein L homeolog ELISA Kit, ANLN ELISA Kit, Anln ELISA Kit, anln ELISA Kit, scra ELISA Kit, anln.L ELISA Kit

Background:

Gene Name: Anillin

Gene Aliases: Scra, Scraps, Actin-Binding Protein Anillin