AIMP1 ELISA Kit

Catalog No. ABIN5651668

Product Details AIMP1 ELISA Kit

Target: AIMP1 (Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1 (AIMP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 125 pg/mL - 8000 pg/mL

Minimum Detection Limit: 125 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1). No significant cross-reactivity or interference between Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) and analogues was observed.

Sensitivity: 46 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for AIMP1

Target: AIMP1 (Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1 (AIMP1))

Alternative Name: Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1 Products)

Synonyms: EMAP2 ELISA Kit, EMAPII ELISA Kit, HLD3 ELISA Kit, SCYE1 ELISA Kit, p43 ELISA Kit, wu:fa95a05 ELISA Kit, zgc:154081 ELISA Kit, AIMP1 ELISA Kit, 9830137A06Rik ELISA Kit, AIMP1/p43 ELISA Kit, Emap2 ELISA Kit, Scye1 ELISA Kit, scye1 ELISA Kit, aminoacyl tRNA synthetase complex interacting multifunctional protein 1 ELISA Kit, aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 ELISA Kit, aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 S homeolog ELISA Kit, AIMP1 ELISA Kit, aimp1 ELISA Kit, Aimp1 ELISA Kit, aimp1.S ELISA Kit

Background:

Gene Name: Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1

Gene Aliases: SCYE1, EMAP II, EMAP2, p43, Small Inducible Cytokine Subfamily E,Member 1, Endothelial monocyte-activating polypeptide II, Multisynthase complex auxiliary component p43

Gene ID: 9255

UniProt: Q12904