Iba1 CLIA Kit
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- Target See all Iba1 (IBA1) CLIA Kits
- Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
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Reactivity
- Mouse
- Detection Method
- Chemiluminescent
- Method Type
- Sandwich ELISA
- Detection Range
- 31.25 pg/mL - 2000 pg/mL
- Minimum Detection Limit
- 31.25 pg/mL
- Application
- ELISA
- Sample Type
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Allograft Inflammatory Factor 1 (AIF1). No significant cross-reactivity or interference between Allograft Inflammatory Factor 1 (AIF1) and analogues was observed.
- Sensitivity
- 1.26 pg/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 2 - 3 h
- Plate
- Pre-coated
- Protocol
- The microplate provided in this kit has been pre-coated with an antibody specific to Allograft Inflammatory Factor 1 (AIF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Allograft Inflammatory Factor 1 (AIF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Allograft Inflammatory Factor 1 (AIF1) level in the sample or standard.,
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Allograft Inflammatory Factor 1 (AIF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Allograft Inflammatory Factor 1 (AIF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all Iba1 (IBA1) CLIA Kits
- Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
- Alternative Name
- Allograft Inflammatory Factor 1 (IBA1 Products)
- Synonyms
- AIF-1 CLIA Kit, IBA1 CLIA Kit, IRT-1 CLIA Kit, IRT1 CLIA Kit, AI607846 CLIA Kit, D17H6S50E CLIA Kit, G1 CLIA Kit, Iba1 CLIA Kit, Bart1 CLIA Kit, iba1 CLIA Kit, mrf-1 CLIA Kit, fa04b11 CLIA Kit, wu:fa04b11 CLIA Kit, AIF CLIA Kit, AIF1 CLIA Kit, aif1 CLIA Kit, allograft inflammatory factor 1 CLIA Kit, AIF1 CLIA Kit, Aif1 CLIA Kit, aif1 CLIA Kit
- Background
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Gene Name: Allograft Inflammatory Factor 1
Gene Aliases: IBA1, IRT1, Daintain, Protein G1, Ionized calcium-binding adapter molecule 1
- Gene ID
- 11629
- UniProt
- O70200
- Pathways
- Smooth Muscle Cell Migration
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