PLAP ELISA Kit

Catalog No. ABIN5651587

Product Details PLAP ELISA Kit

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 125.00 pg/mL - 8000 pg/mL

Minimum Detection Limit: 125.00 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase, Placental (ALPP). No significant cross-reactivity or interference between Alkaline Phosphatase, Placental (ALPP) and analogues was observed.

Sensitivity: 52 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, Placental (ALPP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase, Placental (ALPP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase, Placental (ALPP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alkaline Phosphatase, Placental (ALPP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alkaline Phosphatase, Placental (ALPP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Phosphatase, Placental (ALPP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Alternative Name: Alkaline Phosphatase, Placental (ALPP Products)

Synonyms: ECK0378 ELISA Kit, JW0374 ELISA Kit, psiA ELISA Kit, ALP ELISA Kit, PALP ELISA Kit, PLAP ELISA Kit, PLAP-1 ELISA Kit, ILC ELISA Kit, CTACK ELISA Kit, ESkine ELISA Kit, Akp2 ELISA Kit, alp ELISA Kit, zgc:56672 ELISA Kit, DDBDRAFT_0205491 ELISA Kit, DDBDRAFT_0231570 ELISA Kit, DDB_0205491 ELISA Kit, DDB_0231570 ELISA Kit, ALPI ELISA Kit, Actn2lp ELISA Kit, Alp ELISA Kit, bacterial alkaline phosphatase ELISA Kit, alkaline phosphatase, placental ELISA Kit, alkaline phosphatase, placental type ELISA Kit, C-C motif chemokine ligand 27 ELISA Kit, alkaline phosphatase, liver/bone/kidney ELISA Kit, alkaline phosphatase ELISA Kit, alkaline phosphatase, intestinal ELISA Kit, PDZ and LIM domain 3 ELISA Kit, phoA ELISA Kit, ALPP ELISA Kit, LOC100436725 ELISA Kit, CCL27 ELISA Kit, alpl ELISA Kit, alp ELISA Kit, ALPI ELISA Kit, Pdlim3 ELISA Kit, Alpp ELISA Kit

Background:

Gene Name: Alkaline Phosphatase, Placental

Gene Aliases: PLAP1, ALP, ALPG, ALPPL, GCAP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1