Adenylate Kinase 3 ELISA Kit

Catalog No. ABIN5651437

Product Details Adenylate Kinase 3 ELISA Kit

Target: Adenylate Kinase 3 (AK3)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cerebrospinal Fluid, Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Adenylate Kinase 3 (AK3). No significant cross-reactivity or interference between Adenylate Kinase 3 (AK3) and analogues was observed.

Sensitivity: 0.146 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Kinase 3 (AK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Kinase 3 (AK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Kinase 3 (AK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenylate Kinase 3 (AK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Kinase 3 (AK3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Kinase 3 (AK3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Adenylate Kinase 3

Target: Adenylate Kinase 3 (AK3)

Alternative Name: Adenylate Kinase 3 (AK3 Products)

Synonyms: AK3L1 ELISA Kit, AK6 ELISA Kit, AKL3L ELISA Kit, AKL3L1 ELISA Kit, FIX ELISA Kit, AK4 ELISA Kit, AA407498 ELISA Kit, AI506714 ELISA Kit, AK-3 ELISA Kit, Ak3l ELISA Kit, Ak3l1 ELISA Kit, Akl3l ELISA Kit, ak3l1 ELISA Kit, sb:cb845 ELISA Kit, zgc:64135 ELISA Kit, id:ibd3034 ELISA Kit, wu:fa02c11 ELISA Kit, wu:fc20h08 ELISA Kit, AK3 ELISA Kit, CG6612 ELISA Kit, DAK3 ELISA Kit, Dak3 ELISA Kit, Dmel\\CG6612 ELISA Kit, bs12h03.y1 ELISA Kit, MGC114646 ELISA Kit, ak6 ELISA Kit, adenylate kinase 3 ELISA Kit, Adenylate kinase 3 ELISA Kit, adenylate kinase 3 L homeolog ELISA Kit, AK3 ELISA Kit, Ak3 ELISA Kit, ak3 ELISA Kit, Adk3 ELISA Kit, ak3.L ELISA Kit, ADK3 ELISA Kit

Background:

Gene Name: Adenylate Kinase 3

Gene Aliases: AK3L1, AK6, AKL3L, Adenylate Kinase 3 Alpha-Like 1, GTP:AMP Phosphotransferase AK3, Mitochondrial

Gene ID: 50808

UniProt: Q9UIJ7

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process