AHCY ELISA Kit

Catalog No. ABIN5651426

Product Details AHCY ELISA Kit

Target: AHCY (Adenosylhomocysteinase (AHCY))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Adenosylhomocysteinase (AHCY). No significant cross-reactivity or interference between Adenosylhomocysteinase (AHCY) and analogues was observed.

Sensitivity: 0.061 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenosylhomocysteinase (AHCY). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenosylhomocysteinase (AHCY). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenosylhomocysteinase (AHCY), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenosylhomocysteinase (AHCY) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenosylhomocysteinase (AHCY) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenosylhomocysteinase (AHCY) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for AHCY

Target: AHCY (Adenosylhomocysteinase (AHCY))

Alternative Name: Adenosylhomocysteinase (AHCY Products)

Synonyms: sahh ELISA Kit, PSPTO5068 ELISA Kit, Ahcy ELISA Kit, SAHH ELISA Kit, adoHcyase ELISA Kit, ahcy ELISA Kit, AA987153 ELISA Kit, AL024110 ELISA Kit, CuBP ELISA Kit, D150 ELISA Kit, cb1079 ELISA Kit, hm:zeh1173 ELISA Kit, hm:zeh1364 ELISA Kit, wu:fj67b02 ELISA Kit, adenosylhomocysteinase ELISA Kit, Adenosylhomocysteinase ELISA Kit, S-adenosylhomocysteine hydrolase ELISA Kit, adenosylhomocysteinase S homeolog ELISA Kit, AHCY ELISA Kit, ahcy ELISA Kit, ahcY ELISA Kit, MMAH_RS02605 ELISA Kit, Srot_2217 ELISA Kit, Ndas_3755 ELISA Kit, Deba_0936 ELISA Kit, Palpr_2392 ELISA Kit, Calni_0569 ELISA Kit, LOC100282150 ELISA Kit, Intca_2510 ELISA Kit, Marky_0392 ELISA Kit, Desac_1394 ELISA Kit, Tc00.1047053511229.50 ELISA Kit, Tc00.1047053511589.200 ELISA Kit, Tb11.01.1350 ELISA Kit, LMJF_36_3910 ELISA Kit, MCYG_06244 ELISA Kit, Ahcy ELISA Kit, ahcy.S ELISA Kit

Background:

Gene Name: Adenosylhomocysteinase

Gene Aliases: SAHH, AdoHcyase, S-Adenosylhomocysteine Hydrolase, S-adenosyl-L-homocysteine hydrolase

Gene ID: 191

UniProt: P23526