ADK ELISA Kit

Catalog No. ABIN5651422

Product Details ADK ELISA Kit

Target: ADK (Adenosine Kinase (ADK))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Adenosine Kinase (ADK). No significant cross-reactivity or interference between Adenosine Kinase (ADK) and analogues was observed.

Sensitivity: 0.59 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenosine Kinase (ADK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenosine Kinase (ADK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenosine Kinase (ADK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenosine Kinase (ADK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenosine Kinase (ADK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenosine Kinase (ADK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ADK

Target: ADK (Adenosine Kinase (ADK))

Alternative Name: Adenosine Kinase (ADK Products)

Synonyms: AK ELISA Kit, ADK ELISA Kit, DDBDRAFT_0186795 ELISA Kit, DDBDRAFT_0230174 ELISA Kit, DDB_0186795 ELISA Kit, DDB_0230174 ELISA Kit, ATPADK1 ELISA Kit, F3G5.4 ELISA Kit, F3G5_4 ELISA Kit, adenosine kinase ELISA Kit, adk ELISA Kit, 2310026J05Rik ELISA Kit, 5033405D03Rik ELISA Kit, AI255373 ELISA Kit, AI987814 ELISA Kit, Ak ELISA Kit, adenosine kinase ELISA Kit, ATP:adenosine 5'-phosphotransferase ELISA Kit, Adenosine kinase ELISA Kit, adenosine kinase S homeolog ELISA Kit, ADK ELISA Kit, adk ELISA Kit, Adk ELISA Kit, CNN02130 ELISA Kit, LOC5572668 ELISA Kit, CMU_026370 ELISA Kit, adk.S ELISA Kit

Background:

Gene Name: Adenosine Kinase

Gene Aliases: AK, Adenosine 5'-Phosphotransferase

Gene ID: 132

UniProt: P55263

Pathways: Ribonucleoside Biosynthetic Process