Adenosine A2a Receptor ELISA Kit

Catalog No. ABIN5651416

Product Details Adenosine A2a Receptor ELISA Kit

Target: Adenosine A2a Receptor (ADORA2A)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Adenosine A2a Receptor (ADORA2a). No significant cross-reactivity or interference between Adenosine A2a Receptor (ADORA2a) and analogues was observed.

Sensitivity: 0.06 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenosine A2a Receptor (ADORA2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenosine A2a Receptor (ADORA2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenosine A2a Receptor (ADORA2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenosine A2a Receptor (ADORA2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenosine A2a Receptor (ADORA2a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenosine A2a Receptor (ADORA2a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Adenosine A2a Receptor

Target: Adenosine A2a Receptor (ADORA2A)

Alternative Name: Adenosine A2a Receptor (ADORA2A Products)

Synonyms: ADORA2A ELISA Kit, adora2a.1 ELISA Kit, zgc:194773 ELISA Kit, A2aR ELISA Kit, ADORA2 ELISA Kit, RDC8 ELISA Kit, A2AAR ELISA Kit, AA2AR ELISA Kit, A2ar ELISA Kit, ADENO ELISA Kit, Adora2l1 ELISA Kit, adora2a.2 ELISA Kit, zgc:193802 ELISA Kit, zgc:193822 ELISA Kit, adenosine A2a receptor ELISA Kit, adenosine A2a receptor a ELISA Kit, adenosine A2a receptor L homeolog ELISA Kit, adenosine A2a receptor b ELISA Kit, ADORA2A ELISA Kit, Adora2a ELISA Kit, adora2aa ELISA Kit, adora2a.L ELISA Kit, adora2a ELISA Kit, adora2ab ELISA Kit

Background:

Gene Name: Adenosine A2a Receptor

Gene Aliases: ADORA2, RDC8, hA2AR

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints