APC ELISA Kit

Catalog No. ABIN5651413

Product Details APC ELISA Kit

Target: APC (Adenomatous Polyposis Coli (APC))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Adenomatosis Polyposis Coli Protein (APC). No significant cross-reactivity or interference between Adenomatosis Polyposis Coli Protein (APC) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenomatosis Polyposis Coli Protein (APC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenomatosis Polyposis Coli Protein (APC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenomatosis Polyposis Coli Protein (APC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenomatosis Polyposis Coli Protein (APC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenomatosis Polyposis Coli Protein (APC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenomatosis Polyposis Coli Protein (APC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for APC

Target: APC (Adenomatous Polyposis Coli (APC))

Alternative Name: Adenomatosis Polyposis Coli Protein (APC Products)

Synonyms: dp2 ELISA Kit, dp3 ELISA Kit, fap ELISA Kit, fpc ELISA Kit, xapc ELISA Kit, dp2.5 ELISA Kit, AI047805 ELISA Kit, AU020952 ELISA Kit, AW124434 ELISA Kit, CC1 ELISA Kit, Min ELISA Kit, mAPC ELISA Kit, BTPS2 ELISA Kit, DP2 ELISA Kit, DP2.5 ELISA Kit, DP3 ELISA Kit, GS ELISA Kit, PPP1R46 ELISA Kit, RATAPC ELISA Kit, APC1 ELISA Kit, APC, WNT signaling pathway regulator ELISA Kit, adenomatous polyposis coli ELISA Kit, adenomatous polyposis coli protein, putative ELISA Kit, adenomatosis polyposis coli ELISA Kit, adenomatous polyposis coli L homeolog ELISA Kit, APC ELISA Kit, apc ELISA Kit, Smp_139190 ELISA Kit, Apc ELISA Kit, apc.L ELISA Kit

Background:

Gene Name: Adenomatosis Polyposis Coli Protein

Gene Aliases: DP2, DP2.5, DP3, FAP, FPC, GS, Deleted in polyposis 2.5

Gene ID: 324

UniProt: P25054

Pathways: WNT Signaling, Stem Cell Maintenance, Cell-Cell Junction Organization