AP2M1 ELISA Kit

Catalog No. ABIN5651410

Product Details AP2M1 ELISA Kit

Target: AP2M1 (Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Adaptor Related Protein Complex 2 Mu 1 (AP2m1). No significant cross-reactivity or interference between Adaptor Related Protein Complex 2 Mu 1 (AP2m1) and analogues was observed.

Sensitivity: 0.057 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adaptor Related Protein Complex 2 Mu 1 (AP2m1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adaptor Related Protein Complex 2 Mu 1 (AP2m1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adaptor Related Protein Complex 2 Mu 1 (AP2m1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adaptor Related Protein Complex 2 Mu 1 (AP2m1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adaptor Related Protein Complex 2 Mu 1 (AP2m1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adaptor Related Protein Complex 2 Mu 1 (AP2m1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for AP2M1

Target: AP2M1 (Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))

Alternative Name: Adaptor Related Protein Complex 2 Mu 1 (AP2M1 Products)

Synonyms: AP50 ELISA Kit, CLAPM1 ELISA Kit, mu2 ELISA Kit, Ap50 ELISA Kit, Clapm1 ELISA Kit, cb34 ELISA Kit, Ap2m1 ELISA Kit, dpy-23 ELISA Kit, sb:cb34 ELISA Kit, zgc:55711 ELISA Kit, zgc:85653 ELISA Kit, wu:fa97a10 ELISA Kit, ap2m1-a ELISA Kit, ap2m1 ELISA Kit, zgc:56643 ELISA Kit, AP2M1 ELISA Kit, adaptor related protein complex 2 mu 1 subunit ELISA Kit, adaptor-related protein complex 2, mu 1 subunit ELISA Kit, adaptor-related protein complex 2, mu 1 subunit, a ELISA Kit, adaptor related protein complex 2 mu 1 subunit L homeolog ELISA Kit, adaptor-related protein complex 2, mu 1 subunit, b ELISA Kit, AP-2 complex subunit mu-1 ELISA Kit, clathrin coat assembly protein AP50 ELISA Kit, clathrin coat assembly protein ap50 ELISA Kit, AP2M1 ELISA Kit, Ap2m1 ELISA Kit, ap2m1a ELISA Kit, ap2m1.L ELISA Kit, ap2m1b ELISA Kit, ap2m1 ELISA Kit, cgd8_950 ELISA Kit, PY06523 ELISA Kit, PVX_118455 ELISA Kit, PVX_123590 ELISA Kit, CpipJ_CPIJ003697 ELISA Kit

Background:

Gene Name: Adaptor Related Protein Complex 2 Mu 1

Gene Aliases: AP50, CLAPM1, Clathrin-Associated/Assembly/Adaptor Protein,Medium 1, Plasma Membrane Adaptor AP-2 50kDA Protein, Clathrin Coat Adaptor Protein AP50

Gene ID: 1173

UniProt: Q96CW1

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation, SARS-CoV-2 Protein Interactome