Acylglycerol Kinase ELISA Kit

Catalog No. ABIN5651404

Product Details Acylglycerol Kinase ELISA Kit

Target: Acylglycerol Kinase (AGK)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Acylglycerol Kinase (AGK). No significant cross-reactivity or interference between Acylglycerol Kinase (AGK) and analogues was observed.

Sensitivity: 0.081 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acylglycerol Kinase (AGK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acylglycerol Kinase (AGK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acylglycerol Kinase (AGK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acylglycerol Kinase (AGK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acylglycerol Kinase (AGK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acylglycerol Kinase (AGK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Acylglycerol Kinase

Target: Acylglycerol Kinase (AGK)

Alternative Name: Acylglycerol Kinase (AGK Products)

Synonyms: mulk ELISA Kit, CATC5 ELISA Kit, CTRCT38 ELISA Kit, MTDPS10 ELISA Kit, MULK ELISA Kit, 2610037M15Rik ELISA Kit, 6720408I04Rik ELISA Kit, AI465370 ELISA Kit, Mulk ELISA Kit, RGD1562046 ELISA Kit, fi38e09 ELISA Kit, wu:fi38e09 ELISA Kit, zgc:55462 ELISA Kit, acylglycerol kinase ELISA Kit, acylglycerol kinase S homeolog ELISA Kit, AGK ELISA Kit, agk ELISA Kit, LOC100165096 ELISA Kit, Agk ELISA Kit, agk.S ELISA Kit

Background:

Gene Name: Acylglycerol Kinase

Gene Aliases: MULK, Multiple Substrate Lipid Kinase

Gene ID: 55750

UniProt: Q53H12